Expression and cytotoxic effect of recombinant Newcastle Disease Virus (rNDV) vector expressing enhanced green fluorescent gene in JHH5 cell line

Introduction and Aim: In medicine, the term ‘cancer’ is used to describe a group of disorders defined by uncontrolled cell growth ability invade metastasize other organs. As result body's fight conventional cancer treatments, it essential look for alternative therapeutic avenues. Recent years have seen rise in application reverse genetics techniques including Newcastle disease virus as an oncolytic agent tumor models immunocompetent malignancy. preclinical studies, recombinant NDV (rNDV-GFP) that expresses foreign genes was shown be more effective therapy. The goal this study examine vitro anticancer effects GFP-expressing, genetically modified (rClone3-GFP) strains on JHH5 hepatocellular carcinoma line. Materials Methods: To generate NDV, GFP gene inserted into genome strain (NDVClone30) technology, where now resides region occupied F HN (rNDV-GFP). Using MTT assay, we evaluated rNDV-GFP its potential against cells. Cytopathic were analyzed using fluorescent light microscopy. Results: We observed 96 hours post infection, capable inducing death time-dependent way. Conclusion: Recombinant expressing GFP, demonstrated good results inhibiting growth. Our pave door viral vector treatment liver cancer.